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KMID : 1005220150300030257
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Journal of Embryo Transfer
2015 Volume.30 No. 3 p.257 ~ p.260
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Case of Bacterial Identification in Reproductive Organs of Holstein Dairy Cows with Endometritis or Pyometra
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Choe Chang-Yong
Jung Young-Hun
Cho Yong-Il
Yoo Jae-Gyu
Im Seok-Ki
Kwon Eung-Gi
Seong Hwan-Hoo
Kim Sung-Woo
Cho Young-Moo
Kim Chang-Woon
Kang Da-Won
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Abstract
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Reproductive disorders in cows cause economic loss in livestock farms. Reproductive diseases, such as follicular cyst, luteal cyst, endometritis, pyometra, and repeat breeding cause infertility. Among these diseases, endometritis and pyometra are uterine infections that are leading causes of infertility. This study was performed to investigate the causative agents of uterine diseases using bacterial culture. Bacteria were obtained from the reproductive organs (vagina, uterine cervix, and uterine horn) of dairy cow diagnosed with endometritis or pyometra, and cultured on blood agar. The colonies obtained from cultivation for 24 hours were passaged. To identify the bacteria, the colonies grown in passaged culture Gram stained and applied to an automatic biochemical microbial identification system. Escherichia coli were commonly detected in vagina, uterine cervix, and uterine horn of dairy cows diagnosed to pyometra. The cows having endometritis showed not only Escherichia coli but also Pantoea spp. and Klebsiella spp. strains. Dairy cows that were infected with Escherichia coli in uterus caused mastitis or digestive disease. These results suggest that sanitary feeding and management beforehand are needed to prevent bacterial infections.
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KEYWORD
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endometritis , pyometra , dairy cows , Escherichia coli
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